Research

PROJECTS HANDLED:

1. Investigating the Molecular Mechanism of JMJD6 mediated gene regulation in Breast Cancer(2012- 2013), DBT Biocare Funded.

2. Functional characterization of a novel promoter SNP in hyperbillirubinimea.(2012-13), NIBMG funded.

3. Role of Dendritic Cells in Cytokine mediated Treg-Th17 Cross talk in Malaria. (WB DBT Funded: Sanction no. 22(Sanc)/ BT (Estt)/ RD-20/2013, dated- 07.01.2015.)

4. Role of TGF-beta–SMAD signaling nexus in Treg/Th17 differentiation during experimental Cerebral Malaria, (BRNS funded: project no: 37 (1) / 14/ 54/ 2014 - BRNS / 1740)

5. Role of DC subsets in Treg/Th17mediated immune response during experimental cerebral malaria (Department of Science and Technology (DST) funded, Sanction No: SB/SO/HS-106/2013, dated 21/11/2014)

Research Experience and Techniques:

• Flow Cytometry and Cell Sorting: Cellular isolation by Fluroscence Associated Cell Sorting (FACS) and culture.,In-depth flow cytometric analysis for cell surface CD markers and internal markers.

• Gene/Protein Expression study: Realtime Quantatitaive Polymerase Chain Reaction (q-PCR), Nuclear Protein isolation,Immunoblotting/ Western Blotting, Enzyme linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), Gel Electrophoresis

• Molecular Interaction Study: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), RNA-Protein interaction study, Pull down assay,

• Cellular Assays: Proteasomal Degradation assay, Adhession assay, Phagocytosis assay, ROS measurement, cell viability assays.

• Recombinant DNA Technology: Protein Expression in bacteria, Molecular Cloning, RDT techniques, plasmid isolatuon, Transformation, Transfection.

• Cell culture: Primary, Secondary cell culture, Bacterial Culture.

• Animal Model: Mice

THESIS TITLE: The Impact of Autophagy in Infection-Immunology Crosstalk in Cerebral Malarial Infection

In my doctoral research in Prof. Arindam Bhattacharyya’s laboratory, I studied the immune regulation of autophagy, pathogen processing and degradation in cerebral malarial infection, within splenic dendritic cell subsets (plasmacytoid DC & myeloid DC) and splenic macrophage subsets (Red Pulp & Marginal Zone Macrophages). The work also focused on the pro-and anti-inflammatory pathways, cytokine imbalance induced inflammation, glial cell-mediated inflammation, and regulation of T cells subsets (particularly CD8T cells, Treg, Th17).

1. Nano toolbox in immune modulation and nanovaccines, Mohammad Azharuddin, Geyunjian Harry Zhu, Anirban Sengupta, Jorma Hinkula, Nigel K H Slater, and Hirak K Patra, Trends in Biotechnology [Review Article] (Accepted, In press) (IF: 19.54)

2. Efficacy and Immune Response Elicited by Gold Nanoparticle-Based Nanovaccines against Infectious Diseases, Sengupta, A.; Azharuddin, M.; Al-Otaibi, N.; Hinkula, J. Vaccines 2022, 10, 505. https://doi.org/10.3390/vaccines10040505 [Review Article] (This article belongs to the Special Issue Virus-Like Particle and Nano-Particle Vaccines 2.0) (IF: 4.42)

3. Intranasal Coronavirus SARS-CoV-2 immunization with lipid adjuvants provides systemic and mucosal immune response against SARS-CoV-2 S1 Spike and nucleocapsid protein, Sengupta, A.; Azharuddin, M.; Cardona, M.E.; Devito, C.; von Castelmur, E.; Wehlin, A.; Pietras, Z.; Sunnerhagen, M.; Selegård, R.; Aili, D.; Alamer, A.; Hinkula, J.; Al-Otaibi, Vaccines 2022 Mar, 10(4), 504; https://doi.org/10.3390/vaccines10040504 (IF: 4.42)

4. Partial impairment of late-stage Autophagic Flux in Murine Splenocytes leads to sqstm1/p62 mediated nrf2-keap1 antioxidant pathway activation and induced Proteasome-mediated degradation in Malaria; Sengupta A, Mukherjee S, Ghosh S, Keswani T,Sarkar S, Majumdar G, Das M, Bhattacharyya A.;Microb Pathog. 2020 Jul 18 147:104289. doi: 10.1016/j.micpath.2020.104289 (IF: 3.74)

5. Autophagic Induction modulates Splenic Plasmacytoid Dendritic Cell mediated immune response in Cerebral Malarial infection model; Sengupta A, Keswani T, Sarkar S, Ghosh S, Mukherjee S,Bhattacharyya A., Microbes Infect. 2019 Jun 8. pii: S1286-4579(19)30060-7. doi: 10.1016/j.micinf.2019.05.004 (IF: 2.7)

6. Influence of autophagy, apoptosis and their interplay in filaricidal activity of C-cinnamoyl glycosides; Roy P*, Sengupta A*, Joardar N, Bhattacharyya A, Saha NC, Misra AK, Babu SPS. Parasitology. 2019 May 20:1-38. doi: 10.1017/S0031182019000660 *both authors contributed equally. (IF: 3.24)

7. Impact of autophagic regulation on splenic red pulp macrophages during cerebral malarial infection. Sengupta A, Sarkar S, Keswani T, Mukherjee S, Ghosh S, Bhattacharyya A. Parasitol Int. 2019 Aug;71:18-26. doi: 10.1016/j.parint.2019.03.008. Epub 2019 Mar 11. (IF: 2.23)

8. Differential modulation of glial cell mediated neuroinflammation in Plasmodium berghei ANKA infection by TGF β and IL 6. Sarkar S, Keswani T, Sengupta A, Mitra S, Bhattacharyya A.,Cytokine. 2017 Aug 10. pii: S1043-4666(17)30226-0. doi: 10.1016/j.cyto.2017.07.026 (IF: 3.86)

9. Role of TGF-ß and IL-6 in Dendritic cells, Treg and Th17 mediated immune response during experimental cerebral malaria. Keswani T, Sarkar S, Sengupta A and Bhattacharyya A. Cytokine. 2016 Dec;88:154-166. doi: 10.1016 /j.cyto. 2016.08.034. Epub 2016 Sep 12. (IF: 3.86)

10. Dendritic cells subsets mediated immune response during Plasmodium berghei ANKA and Plasmodium yoelii infection , Keswani T, Sengupta A, Sarkar S, Bhattacharyya A. Cytokine. 2015 Jun;73(2):198-206. doi: 10.1016/j.cyto.2015.02.023. Epub 2015 Mar 16. (IF: 3.86)